MetaboAnalyst是目前比较受欢迎的代谢组学分析工具，主要用于代谢组学数据分析、注释、整合其他多组学数据等。不仅有在线分析同时作者也提供了MetaboAnalystR的R包。
| MetaboAnalyst | MetaboAnalyst | MetaboAnalyst | |||||
|---|---|---|---|---|---|---|---|
| Tools name | 5.0 | 4.0 | 3.0 | XCMS Online | W4M | 3Omics | NOREVA |
| Raw spectral processing | |||||||
| Parameter optimization | +++ | − | − | + | ++ | − | − |
| Supported algorithms | +++ | − | − | ++ | ++ | − | − |
| Resumable analysis | +++ | − | − | − | + | − | − |
| Compound annotation | + | + | − | +++ | ++ | − | − |
| Statistical analysis | |||||||
| Univariate | +++ | ++ | ++ | + | + | − | + |
| Multivariate | +++ | ++ | + | + | +++ | − | ++ |
| Clustering | +++ | +++ | ++ | + | + | − | − |
| Power analysis | √ | √ | √ | − | − | − | − |
| Time-series analysis | √ | √ | √ | − | − | − | √ |
| Biomarker analysis | √ | √ | √ | − | − | − | − |
| Biomarker meta-analysis | √ | √ | − | − | − | − | − |
| Functional analysis | |||||||
| Function analysis (MS peaks) | +++ | ++ | − | ++ | − | − | − |
| Enrichment analysis (compounds) | +++ | + | + | − | − | ++ | − |
| Pathway analysis | +++ | ++ | + | − | − | ++ | − |
| Functional meta-analysis | +++ | − | − | ++ | − | − | − |
| Integrative analysis | |||||||
| Unbiased joint pathway | +++ | + | + | − | − | +++ | − |
| Knowledge-based Network | ++ | ++ | − | − | − | ++ | − |
| Correlation-based Network | ++ | − | − | − | − | − | − |
| Other features | |||||||
| Data normalization | ++ | + | + | − | + | − | +++ |
| Missing value estimation | √ | √ | √ | − | − | − | √ |
| Technical Replicates Merging | √ | − | − | − | − | − | − |
比较MetaboAnalyst (versions 3.0–5.0)和其他工具。其中‘√’ 代表有此功能 ‘-’ 代表缺少功能， ‘+’ 表示支持(‘+’ 的数量代表支持的程度，越多表示更好的支持)
• XCMS online: https://xcmsonline.scripps.edu/.
• Workflow4Metabolomics (W4M): https://workflow4metabolomics.usegalaxy.fr/.
• 3Omics: https://3omics.cmdm.tw/.
• NOREVA: http://idrblab.cn/noreva/.Open in new tab
最新的5.0版本的MetaboAnalyst做了大量的更新：
- Check out our MetaboAnalyst 5.0 paper now available on the 2021 NAR web server issue;
- For multi-omics analysis, try our OmicsAnalyst for various data-driven approaches, or OmicsNet for knowledge-based integration;
- Minor bug fixes based on user feedback (05/31/2021);
- Improved functional meta-analysis data upload page for more flexible data processing (04/27/2021);
- Fixed the issue with synchronized 3D scatter plots for scores and loadings (04/12/2021);
- Upgraded to PrimeFaces 10 and fixed the issue for multi-group figure legend (04/06/2021);
- Added support for merging technical replicates with different mathematical models and QC measures (02/18/2021);
- Updated KEGG IDs in internal compound databases (02/09/2021);
- Added seven tutorials introducing new features in MetaboAnalyst 5.0 (01/15/2021);
官方指导文档：
| Overview of MetaboAnalyst 5.0 | A brief introduction of history, design concepts and key features | 2021-04-15 |
| Raw MS spectral Processing | A detailed tutorial for raw spectral analysis | 2021-04-15 |
| Functional Analysis | A detailed tutorial to guide functional analysis on MS peaks | 2021-04-15 |
| Meta Functional Analysis | A detailed tutorial to guide meta-analysis on MS peaks | 2021-04-15 |
| Joint Pathway Analysis | A brief tutorial to introduce joint pathway analysis | 2021-04-15 |
| Enrichment Analysis | A detailed tutorial to do enrichment analysis | 2021-04-15 |
| Network Exploration | A tutorial to guide network analysis | 2021-04-15 |
| Video Demo Series | A video demo tutorial series | 2021-04-15 |
参考资料：
1.https://www.metaboanalyst.ca/
2.Zhiqiang Pang, Jasmine Chong, Guangyan Zhou, David Anderson de Lima Morais, Le Chang, Michel Barrette, Carol Gauthier, Pierre-Étienne Jacques, Shuzhao Li, Jianguo Xia, MetaboAnalyst 5.0: narrowing the gap between raw spectra and functional insights, Nucleic Acids Research, 2021;, gkab382, https://doi.org/10.1093/nar/gkab382
